Influences of soil microbes with an analysis of an Intercrop of Lolium perenne with Phaseolus vulgaris, and a bacterial seedling inoculation of Lolium perenne with Rhizobium etli (CFN 42)

Charles GallagherGallagher
Advisor - Jan Mikesell

Intercropping with legumes has been a practice for many years because of their beneficial symbiotic relationships which can form between the plant species in the intercrop. This experiment discovered that an intercrop of grasses species (L. perenne) and a legume (P. vulgaris) was in fact, not a beneficial relationship and in fact was inhibitory with respect to the rye grass in above ground dry weight and number of leaves produced by the rye grass. The rye grass, however, did have in increase in height of the largest leaf of the plants intercropped with more common bean, most likely as a means to compete with the legume. These relationships between plants species can be analyzed even further with the analysis of the different bacterium which have been isolated from the roots of the plants. Many bacteria have been analysis and found to be plant growth promoting rhizobacteria (PGPR), which can through different mechanism aid plant growth. This study found that rye grass seedlings, which were not rinsed with bleach and inoculated with CFN 42, had a significantly larger leaf height after 41 days (MS=83.3311, F=3.94, P=0.016860) and 48 days (MS=103.9106, F=3.55, P=0.025191) of growth under green house conditions.